Alan Hassall

Background

About Alan Hassall

Research scientist with 15+ years in animal vaccine development. Provided most of the laboratory support for a major canine vaccine license. Enjoy working with people to get them what they need. Skills: Microbiology: Bacterial/ Viral Growth, Mammalian and Insect Culture, Virus Titration/ Isolation/ Purification, Aseptic Technique, Master Seed Selection and Preparation, Dark Field Microscopy, Biosafety Level 2 and 3 Techniques Molecular Biology: DNA and RNA Purification, DNA Restriction Analysis and Agarose Gel Electrophoresis, DNA Transformation, Transfection, Plaque Purification, Clone Selection, TOPO Cloning for DNA Sequencing, Traditional and Realtime PCR Gels, Blots and Chromatography: SDS PAGE, Western Blot, 2D Electrophoresis, Silver Staining, BCA Assay for Protein Concentration Measurement, Affinity Chromatography, Ion Exchange Chromatography, Size Exclusion Chromatography Immunological Testing: ELISA Development and Optimization, Immunofluorescent Antibody (IFA) Assays, Serological/ Diagnostic Assays, Micro and Ultra Filtration Office Programs: Microsoft Excel, Microsoft Word, Outlook, Skype, and Freezerworks.