Catherine Legrand

Background

About Catherine Legrand

RESEARCH ENGINEER* Sense of responsibility, communication, planning and conducting experiments, coaching experience, project management* Experiences in laboratories ( Emergency - bacteriology - hematology and medical chemistry )-> Main tasks: conducting analysis, calibrations, maintenance of equipments and preparation of reagents in agreement with laboratory procedures.TECHNICAL AND SCIENTIFIC SKILLS - Molecular Biology: PCR, Cloning of DNA, RT-PCR, RNA and DNA extraction, ELISA, Western-Blot, Electrophoresis- Culture of human chondrocytes in alginate beads, culture of cartilage explants- Rheological properties of cartilage: indentomètre MACH-1 (Biomomentum, Canada)- Walking platform for small rodents: CatWalk XT (Noldus, The Netherlands)- Protein purification: affinity chromatography on GE Äkta 10S 2D-LC - GE Akta Explorer 100 AIR - NGC Quest 100- HPLC size exclusion chromatography (SEC) – Alliance e2695 Separations Module (Waters)TRANSVERSAL SKILLS- Work in a multidisciplinary team- Work within an ISO certified environment- Research, selection, processing, classification of information- Writing reports, projects and scientific articles in English- IT skills: MS office (Word, Excel, PowerPoint), software Glims, STATISTICA, GraphPad Prism[Mots clés] research engineer, scientist R&D, dowstream process scientist, osteoarthritis, cartilage, biomarkers, molecular biology, MACH-1, CatWalk XT, Protein purification, osteoarthritis, GMP, life sciences, regulatory affairs, medical devices