Daniel Waller

Background

About Daniel Waller

CERAMISPHERE 5.5 years - Biomedical micro-encapsulation Research Assistant Range of exposure to instrumentation and techniques -Sol-gel and Emulsion Chemistry for micro-particle/nano-particle encapsulation synthesis (including large volume centrifuge and micro-centrifuge sedimentation)-Optical Microscopy (Including Phase Contrast and Fluorescence for live cell culture)-Electron Microscopy (SEM and TEM)-"Malvern" Light Scattering Instruments for particle size distribution and zeta potential measurements - Solid State FTIR (DRIFT) analysis for the qualitative presence of adsorbed protein -Thermal Gravimetric Analysis -Fluorometric Assays (Quantitating Oligonucleotides via Hoechst minor groove binders)-UV-Vis (Colorimetric BCA assay for quantitating proteins)-Reverse Phase HPLC -USP 4, flow-through, automated 'Dissolution Testing' for time-course dissolution parameters of silica micro-particles -Human Tissue Cell Culture (HeLa) involving Aseptic Technique (UV sterilised BSC-II, autoclaving consumables and liquids, preparing sterile FBS media and sanitising 70% EtoH solutions)-SiRNA micro-encapsulation and RNA extractiion from lysed cell culture using RNase free techniques ANSTO Radiopharmaceuticals & Industrials 1 year - Injectable Radiopharmaceutical Production Technician (strict, fast paced, GMP environment)-Strict radiation safety protocols for all procedures -Clean room entrance protocols and monitoring (correct gowning with validation clearance)-Accurate calculation and dilution of primary stock using ion chamber measurements and automated saline dispensers (including addition of preservatives)-Semi-automated dispensing and vial capping -Bulk autoclave operation and final lead pot packaging -QA documentation and transcription of data into SAP system JALCO 1 year - Junior Formulation Chemist and QC officer -Stability and QC testing (varied temp and freeze-thaw cycles, monitoring pH, viscosity and Specific Gravity actives titration and total dry solids)