Daniela Roellig

Background

About Daniela Roellig

As a cell and developmental biology expert with 15+ years of experience in molecular biology, I regularly design and manage research projects for target validation in oncology projects. I believe that nowadays, quantitative approaches and multidisciplinary collaborations are key both to deciphering the molecular mechanisms underlying pathologies and to designing efficient therapeutic solutions. Scientific communication is one of my passions; thanks to my international background I am enthusiastic about sharing my various experiences with collaborators and diverse audiences as well as training scientific staff and students. Lab skills: Molecular Biology and Biochemistry: RT-qPCR, sanger sequencing, cloning, Western blotting, JESS, DNA/RNA/Protein isolation, ChIP, in situ hybridization, Hybridization chain reaction, Microarray, RNAseq Cell Biology: cell culture, siRNA transfection, CRISPR, FACS, DNA transfection, immunofluorescence, automatisation, Cell proliferation and viability assays, automatisation using liquid handlers and automated cell dispensers, digital compound dispenser, compound treatment Microscopy: Operetta, Confocal, AiryScan, spinning disc, light sheet, epifluorescence, live cell imaging Computer skills: Bioinformatics: NCBI Ensembl, BLAST, ClustalW Cloning: SnapGene, Primer3 Image and Data analysis: Excel, Graphpad PRISM, ImageJ/Fiji, Imaris, CellProfiler, Columbus Presentation/ organization: PowerPoint, Word, Adobe Illustrator, GoogleCalendar, GoogleForms, Teams, Zoom, OneNote Courses: intro to R package, statistics, Time management, ImageJ formation, intro to Phyton, French intensive courses, lab safety courses