Darcy Ahern

Background

About Darcy Ahern

I am a Ph.D-level scientist with extensive research experience in the biotech industry, specializing in drug discovery and development. My expertise lies in molecular and cell biology, assay development, gene editing (CRISPR-Cas9), human disease modeling, pharmacology, and new target evaluation. At Intellia Therapeutics, I led pipeline evaluation for novel in vivo gene editing targets, managing cross-functional teams from target validation to preclinical candidate delivery. I have a proven track record of designing and executing complex in vitro studies, analyzing data, and communicating results effectively. My laboratory skills encompass a wide range of techniques, including cell culture (ESCs/iPSCs, primary cells), molecular biology (PCR, RT-qPCR, sequencing), and cellular biology (flow cytometry, microscopy). I am proficient in computational tools like R, FlowJo, and GraphPad Prism. I am passionate about translating scientific discoveries into innovative therapies and seeking opportunities to contribute to cutting-edge research. Laboratory and Computational Skills: • Cell Culture – BSL2 lab training; human stem cells (ESCs/iPSCs); primary human, mouse, and NHP liver and immune cells (hematopoietic, HSCs, CD34+); various mammalian cell lines (HEK293, HepG2, Jurkat, mast cells) • Cellular Biology Techniques – Tissue processing (liver, blood, bone marrow aspirate), flow cytometry, FACS, high-content fluorescence imaging, CRISPR-Cas9 genome editing, EdU proliferation assays • Molecular Biology Techniques – DNA extraction, PCR, genotyping, RNA extraction, gene expression, RT-qPCR, ddPCR, ELISA, protein extraction, Western blotting, next generation sequencing (amplicon-seq, RNA seq), molecular cloning • Computational – bioinformatics (sequencing analysis), statistical analysis (R), ImageJ, GraphPad Prism, FlowJo, e-lab notebooks (ELN), Microsoft Office