Darshini Desai

Background

About Darshini Desai

Co-op, Bristol Myers Squibb and Doctoral candidate at University of Cincinnati studying Genetic Cardiomyopathy using 3D human stem cell derived cardiac organoid model and animal models-Assessing neurotoxicity due to chemotherapeutics induced "chemo fog" for the MS thesis. Grants: AHA predoctoral fellowship: 2 yrs Six plus years expertise in developing 3D cell culture models for preclinical study designs. Proficient in developing various animal, mammalian and human cell culture models. Skilled in screening drug candidates in diseased 3D models to develop potential therapeutics- Expert in studying the pathways related to sarcomere mutations induced cardiomyopathy. Technical skills Cell culture models -iPSC culture, maintenance, expansion and differentiation into cardiomyocytes, endothelial cells and skeletal myoblasts and myotubes - H9C2, C2C12neonatal rat/mouse cardiomyocytes, cardiac fibroblast culture, N27 dopaminergic cell lines-Plasmid transfection, cloning, viral transduction, ELISA, Luminex, MSD, Flow cytometry, Spectrophotometer. Molecular techniques - Protein purification, Western blot, Immunohistochemistry, immunoprecipitation, RT-PCR, qPCR, DNA/RNA extraction and purification, Primer design, Gel electrophoresis. Microscopy skills -Fluorescent Microscopy -Confocal Microscopy Software -Microsoft office -Graph pad Prism -Image J -BioRender -BLAST -Sigmaplot -Endnote -Mendeley