Diego Comparini

Background

About Diego Comparini

Career objective To achieve my ultimate potential in an innovative environment that fully utilises my qualifications, experience and creativity. Technical skills Administration and monitoring cultures of microorganisms. Determintation of cellular concentration through Petroff-Hausser Counter, O.D, dry-weight, CFUs/ml and MPN. Growth curve of a culture. Extraction through solvents. Extraction and purification of RNA from bacteria. Retrotrascription of RNA into cDNA through Reverse transcriptase-Polymerase Chain Reaction. Use of Real-time PCR to monitori genic expression through new primers design and reference genes validation. Use of statistic software to analize data files. Design of new primers for specific genes through primers design software. Calculation of relative expression of genes in a RT-PCR plate through relative expression software tool Seeds sterilization and germination, growth and media preparation of plants, both hydroponic and soil. Dry weight, phenotype monitoring, images scan analysis,extraction of pigments, determination of photosynthesis of plants. Time-lapse photography technique applied to plants and roots growth. Evaluation of electric network activity of roots through the use of the Multi Electrode Arrays system (MEAs). Organization, administration and analysis of large amount of data. Specializzazioni: Key skills Prioritizing workload Computer skills - Microsoft Office: word, excel, access, powerpoint, frontpage, outlook - Rhinoceros 3.0D - Analize.it (statistic software)- R (statistic software)- Rest XL (relative expression Software tool)- CloneManager Suite (primer design software)- Quicktime - GraphPad Prism - Image J Organizing data and files. Languages Italian – Mother Tongue English – Intermediate Japanese – oral Basic/ written Hiragana/Katakana