Felix Grun
Director, Mass Spectrometry Facility at Uc Irvine
Based in Irvine, United States
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Seniority
Director
Department
Science
Location
Irvine
Industry
Higher Education
Company size
14K
Contact information
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f•••••••@uci.edu
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Background
About Felix Grun
Nuclear hormone receptor biology - Advanced molecular cloning strategies - Retinoid/steroid enzyme biochemistry - Tissue culture growth/differentiation assays (cell lines/primary cultures)- High throughput transfection methods - Transcription reporter assays - Receptor-ligand binding assays (BiaCore, FLASHplate, affinity bead)- QRT-PCR - Northern, Southern blotting, cDNA library screens - Immunohistochemistry, Western, IP pulldown assays, FACS - Fluorescence microscopy Endocrine disruptors (focus on environmental obesogens)- Environmental toxicology (field sample collection, assessments)- Natural products characterization - Bioactivity guided fractionation (HPLC w/PDA detection)- Mass spectroscopic identification/quantification (LC-MS QTOF, GC EI-MS, UPLC-MSMS, MALDI-TOF MS)- Metabolomics (Mass Lynx, MarkerLynx software)- steroid/lipophilic metabolites - High throughput screening assays (chemical diversity libraries)- Metabolic syndrome disorders - assays relevant to obesity, diabetes, cardiovascular disease Animal study experience- Murine colony husbandry - Nutritional studies - Glucose stress tests - MRI for fat/water analysis - Fetal exposure models (DOHaD)- Transgenic model analyses (SXR/PXR KO; humanized SXR mice)- Tissue collection (including hepatocyte preps, adipocyte and bone marrow preps)- Xenopus laevis (Fetax) Computational skills- Mac OSX (w/UNIX, X11 experience)- MS Windows XP - Robotics: integrated ThermoFisher CRS setup w/Polara interface - Microsoft Office (advanced Excel user)- Endnote bibliographic manager - Photo/illustration tools (Photoshop, Canvas, Illustrator, Powerpoint)- Scientific graphing/statistical analysis (GraphPad Prism, Instat, ChemDraw)- Analytical packages (Waters Millenium HPLC interface, MassLynx, OpenLynx, MarkerLynx, AMDIS, DICOM viewers (Osirix, MadenaX)- HTS chemical database management/distribution
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