Giulia Rosini

Background

About Giulia Rosini

I have learned and practiced many useful techniques: preparation, maintenance and treatment of immortalized cell cultures; preparation and repropagation of yeast cultures; use of optical microscope and analysis; Cell culture purification; Cellular staining; PCR; Real-Time PCR; Nested PCR; Digital droplet PCR (ddPCR); Digital PCR (dPCR); TaqMan assays; miRNA assays; Custom probe assays; sequencing; DNA and RNA extraction from cells, bacteria, tissue, adult insects and larvae (Hermetia Illucens); miRNA extraction; Gel electrophoresis; Bacterial transformation; Transfection with chemical and physical methods; Construction of Donor vectors for genome-editing; CRISPR Cas9; Plasmid purification; Mutagenesis assays on yeast cultures / bacterial cultures / cell cultures; HPRT assay; Ames test; Pharmacokinetics microbiological analysis; DNA sonication; Chromatin immunoprecipitation (ChIP); Surveyor assay; Manipulation of blood samples, plasma samples and bone marrow biopsy (BOM); Extraction, culturing and manipulation of peripheral blood lymphocyte lines (T-cells); Liquid biopsies, manipulation of Drosophila melanogaster; Analysis of tissue biopsies; Protein extraction from tissue, cells and plants; protein analysis, protein quantification assays; protein purification; HPLC; ELISA; Western Blot; in vitro analysis; ex-vivo analysis; Single Cell Sorting; Flow cytometry; NGS, library preparation; Incucyte using; Lyophilization; Immunohistochemistry (IHC); Immunofluorescence (IF); Immunocitochemistry (ICC); Enzyme assays; Seahorse use, in vivo experience, imaging. Statistical analysis are fundamental in results interpretation.