Holly Bachus

Background

About Holly Bachus

PROFESSIONAL SUMMARY Knowledgeable laboratory professional skilled in multiple facets of research, lab safety, lab management, and animal care. Self-motivated with excellent communication, planning, and problem-solving abilities. Efficient individual with success in independent and team-driven research environments. Dedicated researcher offering over 18 years of research and lab management experience. CRITICAL RESEARCH SKILLS Surgical Skills: Parabiosis, Splenectomy, Advanced knowledge of anesthesia induction and monitoring using injectable and gas anesthesia Multiple organ retrieval and processing (mediastinal lymph nodes, mesenteric lymph nodes, inguinal lymph nodes, popliteal lymph nodes, Peyer’s Patches lymph nodes, cervical lymph nodes, thymus, lungs, spleen, large intestine, cecum, small intestine, kidney) Animal administration (oral gavage, intravenous, IV retro-orbital, IV tail vein, intraperitoneal, intra-tracheal, SQ footpad, SQ ear) Bronchoalveolar lavage collection, Bone Marrow Collection, Blood collection (tail vein, cheek, retro orbital, intra-cardia), Urine collection, Intestinal fecal collection and DNA isolation ELISA, PCR, Cell purification through MACS Flow cytometry-Attune NXT and BD LSRFortessa Maintenance of mouse colony: Breeding, Weaning, Genotyping, Healthcare, Strain development, Euthanasia Extensive knowledge of lab safety regulations/compliance PUBLICATIONS Solid track record of high-impact-factor publications in the top-ranked peer-reviewed journals in the field of Immunology. Sixty percent of manuscripts are published in journals with an impact factor above 20, including Nature Immunology (one publication), Immunity (two publications), and Science Immunology (two publications), among others.