James Davis

Background

About James Davis

A Senior Field Application Scientist with expertise in microplate instrumentation who is addicted to automation with a microscopy problem. An insatiable passion to learn and to teach others to help advance their research. Specialties: laboratory automation, robotics, widefield imaging, confocal microscopy and image processing and analysis, proficient in automation software and hardware assembly and 2D and 3D cell biology workflows. Skills that include Molecular biology: Immunoprecipitation, Prothrombinase Assay’s, Phosphorescence decay, microdissection, FLPC, western/northern blots Column chromatography, ELISA's, wound healing, Fluorescence Polarization, tissue culture, generation of primary tissue cultures, phage library screening, Mouse hybridoma screening, Radioactive labeling of proteins, extensive experience with Adenovirus, Insitu Hybridization, Immunohisochemistry, validation of stable cell lines in functional assays. Organize data and maintain accurate laboratory records. HTS assays:(cAMP,IP3,FLIPR,GTPS), ELISA, Radio Ligand Binding Assays, and scatchard analysis, some experience with malanophores. FP, BRET, TR-FRET, HTRF: cAMP, IPOne. Alphascreen. In-Vivo: Micro surgery, Langendorf hanging heart, MI Model, femoral artery thrombosis model, tail bleeding model, eye bleeds. Computer: PC: Microsoft Word/Excel, PowerPoint, Scion Imaging, Prism, PCI imaging software, Photoshop, and computer hardware upgrades Adobe photoshop, graphic design, management, Microsoft Excel, Microsoft Office, Microsoft PowerPoint, Microsoft word, Prism, Gen5, LHC, BioSpa, RTCA, Seahorse, Precision Power software, research, safety.