Jim Mohan

Background

About Jim Mohan

Immunologist with over 11 years post-graduate experience in cross-disciplinary research with a specific focus in autoimmunity and immuno-oncology. Proven track record of advancing early discovery IO antibody programs through IND filing and into phase 1 clinical trials. Extensive expertise in the application of broad cellular and molecular techniques with demonstrated success in the conception and execution of innovative research projects. Leadership experience in preclinical immune-modulatory drug development programs. Passionate about target discovery, functional characterization and validation of novel therapeutic strategies applicable to autoimmunity, inflammatory disorders and cancer immunotherapy. SKILLS: • Extensive experience with human primary immune cell assays and mouse models of inflammatory diseases • Genome editing - CRISPR-Cas9, homologous recombination, KO/KI gene targeting in ES cells, embryos, and mouse/human cell lines, INDEL analysis • Molecular biology - DNA/RNA isolation, gene cloning, sequence analysis, in vitro transcription, PCR stitching, shRNA, ectopic expression, retroviral/lentiviral transduction, electroporation, lipid nanoparticle mediated delivery • Gene and protein expression analysis - microarray, RNA-Seq, immunoblot, SDS-PAGE • Multi-parametric Flow cytometry – Flowjo, Facsdiva • Microscopy and image analysis - intravital multiphoton and confocal microscopy, in vivo cell tracking, cryo-sectioning and immunofluorescence, Imaris, ImageJ • Recombinant protein expression, purification and analysis • Conventional molecular, biochemical and cellular immunological assays - primary cell isolation, enrichment and cell culture, functional assays, proliferation, antigen specific/superAg T cell assays, MLR, ELISPOT, NK cell activation/degranulation/cytotoxicity assays, ADCC and ADCP assays, cytokine analysis, ELISA/Luminex/MSD, TCR sequencing, bone marrow transplantation, adoptive cell transfer, immunization, hybridoma generation and antibody characterization, small molecule/antibody dosing in mice, survival surgery, tissue harvest and processing • Ligand binding assays, affinity measurements, antibody epitope mapping