John Azeke

Background

About John Azeke

OVERALL CAREER GOALS To apply my skills as a Biomedical Engineer and Materials Scientist to a dynamic and challenging work environment while further developing the qualities of an effective leader. ABOUT MYSELF I am a demonstrated achiever having a broad array of research experiences in basic and translational medical research. I am an ambitious engineer eager to expand my knowledge in the biomedical sciences and have developed many of the essential biological and biochemical skills necessary for success in a research setting. Finally, I enjoy opportunities that allow me to continuously develop my interpersonal skills, especially those involving group management, teaching, and collaboration. TECHNICAL SKILLS -Sol-gel polymer and ceramic chemistry under reduced pressure, toxic, inert, and high temperature conditions -Culture of primary and immortalized human, rat, and chicken hepatocyte, fibroblast, and mesothelial cell lines -Electrophoresis techniques (SDS-PAGE, DNA, Coomassie Blue, Western and Northern blots)-Protein identification and quantification techniques (ELISA, BCA assay, Immunohistochemical localization in excised tissues and fixated cells, Fluorescence and Radioactive labeling and probing of proteins)-DNA and RNA techniques (DNA and RNA isolation and purification, recombinant DNA production, traditional, real-time, and quantitative PCR)-Drug delivery and development techniques (Dose-Response analysis, Transient vs. Sustained delivery of drugs to cells, siRNA and antisense oligonucleotide delivery to cells, MTT Cell viability assay, trans-membrane delivery of drugs by fluorescence microscopy)-Statistical experimental design and analysis (ANOVA, t-test, Linear regression analysis, F-test, Factorial experimental designs, and Surface-Response (process optimization) experimental designs) Specialties-Sol-gel polymer and ceramic chemistry -Culture of primary and immortalized cells -Electrophoresis techniques (SDS-PAGE, DNA, Coomassie Blue, Western and Northern blots)-Protein identification and quantification techniques -DNA and RNA techniques -Drug delivery and development techniques -Statistical experimental design and analysis