Jui Tsai

Background

About Jui Tsai

Diagnostic markers (nucleic acid and protein) quantification • Pre-clinical oncologic drug MOA and clinical disease state characterization through protein and nucleic acid quantification. • Gene expression quantification: qPCR, PCR and Next Generation Sequencing (TaqMan, SYBR Green and Ion Torrent). • Protein localization, quantification and oligomerization determination in pre-clinical model: proximity ligation assay (PLA). • Work flows customization and improvement: Next Generation Sequencing sample quality controls and automation. Protein biomarker characterization • Protein characterization with in vitro biochemistry approach: protein folding, digestion, oligomeric state and interaction assessment. • High throughput protein assay and ddrug target screen: ELISA, yeast-two-hybrid and synthetic lethal. • Protein quantification and localization from liquid biopsy and tissue samples: TaqMan protein assay, ELISA and PLA. • Recombinant protein sample preparation: Expression plasmid/vector construction, protein expression, column purification and end product storage. New technologies import and integration • New technologies introduction, education, training and SLP generation. • Quantitative biology assays designed and customized. • Data validation and integration across various platforms and different laboratories. • Collaboration with manufacturers and inter-groups to make sure the performance of new technologies meet teams expectations in timely manner.