Julia Lerner

Background

About Julia Lerner

Research Scientist with extensive experience in biomedical research. Solid background in Cell Biology, Molecular Biology, Genetics. Significant research projects utilizing expertise in a wide range of basic and advanced research techniques, such as cell-based assays, immunohistochemistry, molecular biology, extracellular vesicles, pre-clinical mouse models. Easily and precisely assimilates, reviews, analyses, and presents scientific data. Highly organized, collaborative, and resourceful problem solver. Key Strengths: • Drive for Results • Scientific Rigor • Out-of-the-box Thinking • Self-Motivation • Strong Project Management Skills • Excellent Presentation Skills Expertise: 1. Cell Culture (cultivating of established cell lines and optimization of culture conditions of primary cells (hMSCs); protein expression in cell lines (transient transfection); cell-signaling assays, reporter-gene assays, ICC, PLA; extracellular vesicles isolation, quantification and characterization; viral gene transfer; embryonic organoid primary cultures) 2. Molecular Biology (DNA/RNA isolation; PCR/qRT-PCR; molecular cloning; CRISPR genome editing) 3. Histology (tissue embedding (FFPE, frozen); sectioning (cryostat, microtome); immunohistochemical/ immunofluorescent staining, ISH, RNA Scope, protein binding; laser microdissection) 4. Protein Biochemistry (Western Blot, WB Arrays; Co-Immunoprecipitation; ELISA, multiplex assays; flow cytometry) 5. Microscopy (highly skilled in brightfield and fluorescent microscopy; Optical Projection Tomography) 6. In-vivo (extensive experience with preclinical mouse models; mouse handling, breeding; injections: IP, oral gavage, IV, HDD, SC tumor engraftment; animal autopsy, embryo microdissection, retinal dissection) 7. Software (GraphPad Prism, Benchling,MacVector, Bulkviewer, MS Powerpoint, Adobe Photoshop, Image Analysis: HALO, Amira, ImageJ)