Lorenz Eing
Research Assistant at The Julius Maximilians University Of Würzburg
Based in Würzburg, Germany
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Seniority
Staff
Department
Science
Location
Würzburg
Industry
Higher Education
Company size
3.4K
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l•••••••@uni-wuerzburg.de
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Background
About Lorenz Eing
How is it possible, that errors in single cells result in total collapse of an organism? Why is there no working therapy against different types of cancer? I wanted an answers- and started studying biology at the JMU Würzburg- resulting in having more and more questions. I successfully graduated with the Master of Science 2017. I wrote my Bachelor thesis at the department of Clinical Neurobiology in 2015, my Master thesis at the Department of Biohpysics and Biotechnology in 2016. From January until June 2017, I was studying in Grenoble, France. In February 2018 I started working at the University Hospital Würzburg as a research assistant in the cell laboratory for Radiotherapy at the University Clinic Würzburg. In January 2020 I started my PhD as a pipeline developer in early drug dicovers in the group of Prof. Dr. Elmar Wolf at the Institute for Biochemistry and Molecular Biology at the Julius-Maximilians Universtiy Würzburg. Skills in laboratory: Cell Biology: Human/murine cell culture (adherent and suspension cancer cells, primary murine cells) • Luciferase-based assays (endpoint and kinetic) • Co-Immunoprecipitation • Gradient centrifugation • Cell viability and colony formation assays • Flow cytometry • Transfection • Incucyte • Single- and collective cell migration (2D) • Laboratory safety level S1 Proteomics: SDS-Page/Immunoblotting • Immunoprecipitation • Mass spectrometry (whole cell and immunoprecipitation) Cell Engineering: DNA and RNA purification • PCR • Restriction and ligation • Lentivirus production/handling • Laboratory safety level S2 Animals: General mouse handling • FELASA B certificate • Organ preparation • purification of T-cells (spleen and lymph nodes) • purification of keratinocytes (skin newborn and tail) Computational: Microsoft Office (Excel, Word, PowerPoint) • GraphPad PRISM • Origin • FlowJo • ApE software • Snapgene viewer • DepMap data analysis • Genome browser • Affinity Designer • Blender • Endnote • R Studio You want to discuss about experiences? Don't hesitate to contact me! With best regards, Lorenz
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