Mithun Roy

Background

About Mithun Roy

Experimental skills: Design and synthesis of organic ligands (compounds) and the corresponding metal complexes, purification of the compounds and the various techniques for crystallizing the compounds using Sleck Techniques.# Spectroscopy: IR, UV-visible, Fluorescence, Time-resolved fluorescence, Circular Dichroism, NMR spectroscopy# Electrochemistry: Cyclic voltametric analysis# Magnetochemistry: In operating and handling of the 300 Series Lewis Coil Force Magnatometer.# X-raycrystallography: Data collection using Bruker SMART APEX CCD diffractometer and data refinement using SHELXTL, SHELX, WINGX and other crystallographic softwares.# DNA and protein binding Study: Binding of small molecules with DNA or protein by emission spectroscopy, absorption spectroscopy, circular dichroic spectral measurements, DNA melting studies, viscosity and electrochemical methods, Isothermal Titration Calorimmetry.# DNA and protein cleavage study: Studies on cleavage of plasmid supercoiled DNA by different coordination complexes by oxidative or by hydrolytic pathway using agarose gel-electrophoresis technique. Protein cleavage study by PAGE.# Cancer cell biology: Experience on animal cell culture and cell viability assays for in vitro screening of organic molecules and metal complexes. Studies on photoactivated anticancer activity of drug molecules (organic or inorganic metal complexes) to different cancer cells. Experience on fluoroscence assisted cell cycle analysis (FACS), cellular imaging using fluoroscence and confocal microscopy. Anticancer activity of the metal complexes or organic compounds have been carried out in the laboratory of Prof. Anjali A. Karande at the department of Biochemistry, IISc.# Computer language: Windows, LinuxSpecialties: Research on Metallodrugs