Pranti D.

Background

About Pranti D.

Hands-on experience with SPR instruments, including Alto and OpenSPR-XT. I conducted training sessions for new users, covering instrument operation, software use, and experimental workflows. Represented Nicoya at industry events, such as ACS Bio, by managing booths and engaging with the scientific community. Pre-Sales Support: Collaborated with sales teams to deliver product demonstrations and presentations to prospective customers. Conducted in-house sample testing for customers and effectively communicated results. Post-Sales Support: Ensured smooth onboarding for customers, provided training on data analysis, and guided users in interpreting kinetic and affinity parameters. Expertise: Chromatography: Proficient in downstream instruments (HPLC, SEC-MALS, SEC, UV), processes (TFF, UF/DF), antibody screening, data analysis, SOP development, and technology transfer to CDMOs. Hands-on experience with chromatography systems such as ÄKTA Pure, Avant, Pilot, and NGCs. Advanced Analytical Tools: Skilled in GCMS and GPC, with a passion for acquiring new technical skills. Protein Purification and Characterization: Experience with SDS-PAGE, Multiplex Western Blot, UV-VIS, TFF, UF/DF, BioAnalyzer, Mass Spectrometry, Lyophilization, and SpectraMax i3x. Assay Development: Expertise in developing assays for protein-protein and antigen-antibody interactions (kinetics) using SPR and BLI instruments. Skilled in fluorescence polarization, ELISA development, coagulation assays, and high-throughput resin screening in 96-well plates. Technical Proficiency: Familiar with tools such as Jira, Confluence, Propel, MS Office Suite, Agilent ELN, Salesforce, Power BI, and DOE software (Cytiva DOE and JMP). Competencies: Laboratory setup, equipment onboarding, cross-functional collaboration, LIMS, quality assurance, strategic planning, and Lean Six Sigma certification (LinkedIn). Molecular Biology: Proficient in gene construct design (PCR, Gibson assembly, and site-directed mutagenesis), qPCR, DNA/RNA extraction from yeast and E. coli, fluorescence microscopy, and microfluidization. I have also led cross-functional teams across EHS, Marketing, Sales, and R&D (projects such as purification of coagulation factor for diagnostic kit, screening antibodies from mouse & rabbit serums, pilot scale purification with research scale equipment), conducted intern training programs, collaborated with IT teams for instrument integration, and delivered instructional sessions on new technologies.