Rajeshwari Chellappan

Background

About Rajeshwari Chellappan

SUMMARY: Dedicated and hypothesis-driven scientist with a robust background in neuroscience, oncology and immunology, specializing in both in-vivo and in-vitro techniques with 8 years of experience in conducting and leading multidisciplinary research leading to several publications, grants and awards. SCIENTIFIC COMPETENCIES: Neuroscience and Immuno-oncology: 7-color flow cytometry (Attune), FACS (DIVA and Symphony) and single cell sorting (WOLF G2); Primary and cancer cell line culture; miRNA isolation and amplification; PCR, RT-PCR, cloning; ELISA and MSD-multiplex; BCA assays, Western Blotting and immunoprecipitation; immunostaining (IHC and ISH) and confocal microscopy; CAR construct development and testing; RNA-seq and NGS; SnapGene, Graphpad Prism, Flowjo and JMP experienced user In-vivo: Models of neurodegeneration, keratitis, lung infections; Small-molecule inhibitors and therapeutic drug evaluation; mice injections, blood/tissue collection, sectioning, necropsy, sample preparation and processing, behavioral testing Managerial: GDP on Benchling; QA/QC of assays, instrument calibration; End-user training; Developed lab SOPs, compliance and safety in accordance to IACUC/EHS; New recruit on-boarding and training