Susanne Bryde

Background

About Susanne Bryde

Biochemistry: ELISA; SDS-PAGE and immunoblotting;(NF)AAS ((non flame) atomic absorption spectrometer); spectrophotometry; fluorescence and radioactive labeling; protein-purification (membrane-proteins, enzymes, antibodies, TNF); BCA; Bradford; phosphorylation assays (32P), isolation of actin (frog oocytes) Cell biology: Baculovirus mediated protein expression (Sf9 cells); mammalian cell culture, bacteria culture, yeast culture, preparation of stably transfected cell lines; sterile techniques; transfection and transformation; preparation of microscopy samples (live cell imaging and fixed); confocal microscopy, live cell imaging; FACS-analysis; cytotoxicity assays; Electromobility Shift Assay, preparation and staining of tissue samples Nanobiotechnology: preparation of platinum based Nanocapsules; immobilisation of cytokines on nano-particles; directed and spatially restricted stimulation of cells with cytokine coated particles; Molecular biology: Cloning, transformation; preparation of competent bacteria; PCR; RNAi; Supervising/teaching experience; international experience; management training (6 weeks) Specialties:(confocal) fluorescence microscopy; cell culture; languages: German (native), English (excellent), Spanish (good/fluent), French (good/fluent), Dutch (excellent)