Syed Aftab

Background

About Syed Aftab

1.Operate and troubleshoot laboratory equipment’s 2.Practice good judgement in dictating what dilutions are appropriate for certain samples 3.Responsible for keeping detailed and quality notes and maintaining accurate calculations of Experiments. 4.Deep quality control checks, calibration and technical troubleshoot. 5.Follow Sterile and aseptic methods during transport of clinical samples. 6.Responsible for conducting experiments using mediums like flow cytometry, mammalian cell culture, cell harvest and cell counting. 7.Prepare gels for western blot, prepare buffer solutions, growth medium and aliquots for cell culture 8.Protein detection techniques and experiments 9.Expert in growing Spheroids (3d cell culture) 10.Western blot, preparing of buffers, Detecting of proteins. 11.Collected, labeled and stored lab samples according to laboratory specifications. 12.Writing Presentation protocols in detail. 13.Collaborated with other PIs to learn new techniques and opportunities. 14.Worked closely with team members to deliver project requirements, develop solutions and meet deadlines. Clinical Research 1.Lab health and safety practices 2.Lab Equipment management Equipment maintenance (daily, weekly, monthly, annual, preventive maintenance) Calibration Validation IQ, OQ, PQ 3.Focus on Isolators Focus on isolator digital system Calibration operation and control 4.Understand GMP and stem cell processing GMP Environment Cleanroom accordance to ISO-14644 and ISO-14698 EU Guidelines Good Manufacturing Practices Operating controlled rate freezer for manipulation 5.Brief about training on samples manipulation (Stem Cells) 6.Understanding the purpose and use of Canister. Designing Customizing Constructing a Canister. 7.Good Manufacturing Practice/GMP-grade Mesenchymal Stem Cell extraction and subsequent expansion (from human adipose tissue using automated processing system) 8.pharmaceutical isolator use, processing, calibration and control of environment verifications for ambient parameters (i.e, temperature, humidity, particle and gas monitoring) 9.automated production of regenerative products (i.e, human plasma rich in Fibrin and human fibrin sealants) 10.understanding of Human Bone Marrow collection practice and techniques by developing a video presentation and participating in all the private Bone Marrow Transplantation education sessions. 11.knowledge and experience in market search and procurement processes in the laboratory, inventory management, and lab health & safety.