Thomas Cardon

Background

About Thomas Cardon

Scientific Research and Development (R&D) Professional with extensive experience in the biotechnology industry. Specializes in the downstream process of development involving protein purification and filtration (bench scale to early-stage process development) practices. Proven ability to design, deliver, maintain and support innovative, integrated research and developmental solutions. Successful in leading, influencing and motivating both direct reports and other team members. Verifiable track record of delivering multiple projects on or under deadline. Well adept in overexpression of recombinant proteins, protein engineering, proteomics, and/or analytical development. Thrives in team focused environments but is open to other collaborative and/or independent responsibilities and/or duties. Willing to relocate and available for immediate hire (2-week notice). – KEY ACCOMPLISHMENTS– • Led the successful proposition, design, writing, and optimization of trypsin digestion for a new class of insulin analogs- Decreased digestion times to 1-7 days (formerly several weeks to 100 days)- Increased overall process yields from 5% to as much as 50% with significantly increased purities. • Proposed the use of tangential flow filtration (TFF) in order to solve any existing process development issues- Shortened production times from 2 weeks to 1 week- Eliminated the need to lyophilize sample between steps; decreasing need for several sub-batches to one. • Successfully designed and constructed a suction filtration manifold to enable hydrophobic interaction chromatography (HIC) purification of four samples simultaneously; ultimately increased throughput and load volumes from 1L to 20L. • Proposed, designed, and performed proof-of-concept experiment during down time to convince Graduate Research Advisor to incorporate a new technique into our research, which resulted in a publication in a peer-reviewed journal.