Tianjun Sun

Background

About Tianjun Sun

Proficiency in the field of biochemistry with years of postdoctoral experience in Dr. Natalie Strynadka lab at University of British Columbia • Profound knowledge in biochemistry, organic chemistry, general chemistry and biology • Solid teaching and demonstration skills strengthened as a teaching assistant with a wide mood of students in University of Calgary • Profound course planning and class management skills developed through years of experience as a Sunday School teacher • Excellent communication, team-work and organization skills developed with years of lab experiments and volunteering experience. • A dedicate Christian who has passion to share gospel to young people • Solid Skills in MS Office: Word, Excel, PowerPoint, and Linux • Fluency in Mandarin in both speaking and writing • Advanced skills in structural biology with more than ten publications on varied SCI journals: o Protein X-ray crystallography protein crystallization, data collection, structure determination and refinement (using CCP4, Phenix and Coot), structural analysis (using PyMOL and Coot). o Electron microscopy preparing grids for negative-stain EM and cryo-EM, using RELION for single-particle processing. o Molecular biology PCR, cloning, site-directed mutagenesis and construct design. o Protein purification (including membrane protein) protein expression in bacterial system or cell-free system, protein purification (ion-exchange, gel filtration, and affinity chromatography) using FPLC. o Protein Characterization isothermal titration calorimetry (ITC), enzyme kinetic (using spectrophotometer and plate reader), thermal shift assay (Stargazer), circular dichroism (CD), cross-linking method, analytical ultracentrifugation, western blot, radioactive phosphorylation assay,