Tinya Fleming

Background

About Tinya Fleming

SUMMARY OF QUALIFICATIONS • Development and optimization of new assays, techniques and protocols including Verification and Validation studies for FDA submissions. • Design transfer of product into production involving collaboration with many different industry departments including operations, marketing, quality control, and software. • Experienced in managing multiple collaborations and projects involving a wide range of scientific disciplines such as molecular biology, microbiology, chemistry, biophysics, bioengineering, and bioinformatics. • Highly motivated and dedicated scientist with considerable presentation and writing experience who is an effective leader, team member, and individual contributor • Strong critical thinker with a process-oriented approach to designing experiments and a data-driven approach to analysis and interpretation of results. SUMMARY OF RESEARCH SKILLS • Next Generation Sequencing: MiSeq, HiSeq, NextSeq • Genetic & Molecular Biology: PCR, rtPCR, DNA and RNA purification, molecular cloning, genetic manipulation of bacteria, yeast, nematodes and flies, transduction, transformation, southern blotting and RNAi. Utilized next-generation sequencing to acquire whole genome sequence of novel microbes for bioinformatics studies. • Microscopy: Fluorescence, immunofluorescence, confocal, time-lapse, DIC, TIRF, PALM imaging techniques. • Microbiology & Cell Biology: diverse bacterial culture techniques and cell quantification assays (ex: MIC, flow cytometry) • Biochemistry: protein expression and purification, extraction of natural products from microbes using HPLC and MALDI mass spectrometry, and Western blots. • Computer Software: Microsoft Office, JMP, FileMaker Pro, MATLAB, Adobe Creative Suite, ImageJ, molecular genetics software (ex: Sequencher, ApE), bioinformatics software (ex: BLAST, NCBI, CLC Genomics Workbench)