Vasanth Reddy

Background

About Vasanth Reddy

A highly motivated and target-driven professional with 8 years of expertise in molecular and cell biology research, specializing in developing oligonucleotide candidates (aptamer, siRNA, saRNA) for immuno-oncology, neurodegenerative diseases, liver indications, and targeted cancer therapy. • Proficient in leading multifaceted projects with strong multitasking and problem-solving abilities. Skilled in chemical synthesis and analytical characterizations of oligonucleotides. Solid background in cell- and protein-based chemical biology assays. Extensive exposure to nanoparticle/polymer analysis and animal works. • A proactive leader in inspiring projects, maintaining lab and mentoring young scientists. Proven track record of independent and collaborative research, resulting in 2 patents and 21 publications in high-impact journals. SKILLS • Highly proficient in chemical synthesis: DNA synthesis and purification, RNA in vitro transcription, Surface modification, Aptamer development, Polymerization, Conjugation chemistry, Lipid/Nanoparticle modification. • Highly experienced in analytical and chemical biology techniques: HPLC, UV-Vis, DLS, Fluorescence spectrometry, Octet, SPR, TEM DNA/RNA/Protein extraction, Real-time PCR (RT-PCR), PCR, ELISA, Colorimetric protein assays, Gel electrophoresis, EMSA, MSD, FRET, Western blotting, Size-exclusion/Affinity chromatography, Systematic Evolution of Ligands by EXponential Enrichment (SELEX), Electrochemiluminescence (ECL), Pseudovirus neutralization assay. • Strong expertise in cell-based studies: Cell culture, Cell transfection, Stable cell line generation, Cell viability assays (MTS/MTT), Human and mouse primary hepatocytes (PHH, PMH), Hepatocyte isolation, Flow cytometry (FACS), Confocal microscopy. • Hands-on experience in nanoparticle and polymer chemistry, and animal model studies (animal anatomy, Cryostat, in-vivo imaging).