Vinay Kumar Mehra

Background

About Vinay Kumar Mehra

I am self-directed research & development professional with comprehensive accomplishments and knowledge in CRISPR/Cas9 gene editing, cell culture molecular biology, and Malaria. I also have work experience on COVID-19 testing in the VRDL lab and have more than three years’ work experience as Senior Research Fellow and Research Assistant in different research institutes. Currently pursuing Ph.D. in Animal Biotechnology. I have also qualified for the following national exams- 1. Council of Scientific and Industrial Research - NET and Junior Research Fellowship (JRF) 2. Department of Biotechnology -JRF 3. Indian Council of Medical Research - JRF 4. Indian Council of Agricultural Research – SRF and Nation Eligibility Test (NET) Domain Expertise: • Genome editing through CRISPR • Mammalian cell culture, stem cell culture, microbial culture • Transfection in mammalian cells • COVID-19 testing • Gene cloning and bacterial Transformation, • Recombinant plasmid preparation • Molecular Biology: RNA/DNA Isolation, PCR, Real Time-PCR, Agarose Gel Electrophoresis, SDS-PAGE. • Cloning Strategies: Competent Cells Preparation, Plasmid Extraction, and plasmid Cloning • Biophysical Techniques: Spectroscopy, Light Microscopy, fluorescence microscopy • Biochemical Techniques: ELISA, Protein Estimation Assay • Computational Biology: BLAST, MEGA-7, Primer 3 plus, UCSC Genome Browser, NCBI Tools, RNA-Seq Analysis, Sanger sequencing, Next Generation Sequencing • Plant tissue culture