Yingying Han

Background

About Yingying Han

I am working on spatial biology. My experience in biology list below:1. Six years of experience in frozen section and formalin fixed paraffin embedded (FFPE) section.2. Two years of experience in spatial assay Codex3. Experience in spatial assay stereo-seq for fresh frozen samples and stereo-seq for FFPEsamples.4. Six years of experience in ATAC-seq and single cell RNA-seq.5. More than ten years of experience in molecular biology with expertise in DNA/RNA extraction, molecular cloning,mutagenesis, plasmid construction, western blotting and RT-qPCR.6. Six years of experience in 3D organoids and mammosphere culture in different conditions. And co-culture immune cells with mammary stem cells.7. Six years of experience in Light sheet 3D imaging, RNAscope, In situ hybridization, immunofluorescence.8. Six years of experience in multi-color flow cytometry.9. Seven years of experience in NGS libraries preparation: Bulk RNA-seq, Single cell RNA-seq.10. Five years of experience in chromatin immunoprecipitation and ATAC-seq.11. Six years of experience in isolation and culture of primary stem cells. Culture and passage cell lines: 293T,breast epithelial cell line MCF10A, and human breast cancer cell line MDA-MB-231.12. Five years of experience with lentivirus and adenovirus vector production and six years of experience in lipofectamine transfection.13. Ten years of experience with confocal microscopy.14. Six years of experience in animal surgeries such as mouse mammary gland transplantation assay.15. Software: GraphPad, ImageJ, Prism, Photoshop, Illustrator, Leica Microsystems softwares, R Studio.16. Independent critical thinking and scientific achievement in research.17. Extensive experience mentoring junior researchers.18. Working in collaborative teams & have strong publications record in top tier journals.