Ivan Budyak
Senior Director, Analytical Development - Biophysical Characterization at Eli Lilly And Company
Based in Indianapolis, United States
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Seniority
Director
Department
Science
Location
Indianapolis
Industry
Pharmaceutical Manufacturing
Company size
58K
Contact information
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i•••••••@lilly.com
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Background
About Ivan Budyak
Protein expression • PCR, cloning, expression of soluble and membrane proteins in bacteria, yeast, insect and mammalian cells (stable and transient systems), experience in large-scale (30 and 200 L) fermentation of bacteria Protein purification • IEX, HIC, RPC, analytical and preparative SEC, Ab- and Me-affinity chromatography • FPLC systems Äkta Prime, Explorer 100 and Purifier 10, Bio-Rad BioLogic Protein biochemistry • Western blot, SDS-PAGE, native PAGE, pull-down (IP and co-IP), chemical cross-linking, multiple activity assays Protein biophysics • steady state and time-resolved UV-Vis, FTIR, CD, fluorescence • SAXS, SANS, DLS, X-ray data collection (DESY and ESRF synchrotrons, in-house rotating anode) • protein/peptide identification by MALDI-TOF and LC-MS Bioinformatics • Python programming, structure and sequence database analysis
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