Sushma Sharma

Background

About Sushma Sharma

More than 18 years of research experience in microbiology, molecular biology with emphasis on yeast genetics and genomics. Extensive research and technical experience in:Understanding ribonucleotide and deoxyribonucleotide metabolism and DNA damage/S-Phase checkpoint activation in yeast Saccharomyces cerevisiae. Quantification and analysis of dNTP and NTP pool imbalances in various yeast mutants as well as in mice embryos, tissues and mammalian cell lines through nucleotide extraction and its determination by HPLC and LC-MS.Studying cell cycle progression by cell synchronization in Saccharomyces cerevisiae using flow-cytometry. Analysis of mutation rate and mutation spectra in yeast Saccharomyces cerevisiae.Understanding synthetic genetic interaction between various yeast mutants by yeast crossings and tetrad analysis.Construction of genomic disruptions or gene knockouts/deletions in yeast strains.Understanding DNA damage checkpoint activation by SDS electrophoresis and Immunoblot analysis. Research Interests: Study on DNA damage/repair and understanding the molecular mechanisms underlying regulated cell cycle in eukaryotes. Various modes of signal transduction pathways in prokaryotes and eukaryotes. Understanding host-pathogen interactions in yeast, bacterial and viral diseases.